ABSTRACT
OBJECTIVE@#To evaluate antidiabetic activity of chloroform extracts of Acacia arabica bark, Benincasa hispida fruit, Tinispora cordifolia stem, Ocimum sanctum areal parts and Jatropha curcus leaves.@*METHODS@#The chloroform extracts of Acacia arabica bark, Benincasa hispida fruit, Tinospora cordifolia stem, aerial part of Ocimum sanctum and Jatropha curcus leaves were evaluated at different doses (250 and 500 mg/kg body weight.) for antidiabetic potentials in alloxan induced diabetic albino rats. The extracts were administered for two weeks in different groups whereas tolbutamide (80 mg/kg body weight) was used as reference standard throughout study.@*RESULTS@#The result of present study showed test compounds significantly decreases elevated level of serum glucose and also caused to reverse the cholesterol, triglyceride, HDL and LDL values when compared to untreated diabetic rats.@*CONCLUSIONS@#Our finding indicates that different test extracts were able to ameliorate the derangements in lipid metabolism caused by diabetes mellitus in alloxan induced diabetic rats towards normal level.
Subject(s)
Animals , Male , Rats , Blood Glucose , Cholesterol , Blood , Diabetes Mellitus , Drug Therapy , Disease Models, Animal , Hypoglycemic Agents , Pharmacology , Lipoproteins, HDL , Blood , Lipoproteins, LDL , Blood , Plant Extracts , Pharmacology , Plant Leaves , Chemistry , Plant Stems , Chemistry , Plants, Medicinal , Chemistry , Triglycerides , BloodABSTRACT
OBJECTIVE@#To isolate and characterize antidiabetic component (bioactivity-guided fractionation) from hydro alcoholic extract of Ocimum sanctum (O. sanctum) aerial part.@*METHODS@#Ten fractions (F1-F10) were isolated from hydro alcoholic extract of O. sanctum aerial part by column chromatography. All the fractions F1 to F10 were screened for antidiabetic activity in alloxan induced diabetic rats by estimating serum glucose level and lipid parameters. The isolated bioactive component was elucidated on the basis of extensive spectroscopic (UV, IR, MS, (1)H and (13)C NMR) data analysis.@*RESULTS@#The bioactive fraction (F5) was found to be potent antidiabetic by ameliorating glucose and lipid parameters (total cholesterol, triglycerides, low and high density lipoprotein cholesterol). The extensive spectroscopic data analysis reveals that, the isolated bioactive compound elucidated as tetracyclic triterpenoid [16-hydroxy-4,4,10,13-tetramethyl-17-(4-methyl-pentyl)-hexadecahydro-cyclopenta[a]phenanthren-3-one].@*CONCLUSIONS@#Our present study concluded that, tetracyclic triterpenoid isolated from aerial part of O. sanctum has a great anti-diabetic potential.
Subject(s)
Animals , Male , Rats , Biological Assay , Methods , Blood Glucose , Chemical Fractionation , Chromatography, Liquid , Diabetes Mellitus, Experimental , Drug Therapy , Hypoglycemic Agents , Chemistry , Pharmacology , Lipids , Blood , Ocimum , Chemistry , Plant Extracts , Chemistry , Pharmacology , Plant Structures , Chemistry , Triterpenes , Chemistry , PharmacologyABSTRACT
In India, indigenous remedies have been used in the treatment of diabetes mellitus since the time of Charaka and Sushruta. Plants have always been an exemplary source of drugs and many of the currently available drugs have been derived directly or indirectly from them. The ethnobotanical information reports that about 800 plants may possess anti-diabetic potential. Out of several Indian medicinal plants 33 plants were reviewed. The most effective antidiabetic Indian medicinal plants are Acacia arabica, Aegle marmelose, Agrimonia eupatoria, Allium cepa, Allium sativum, Aloe vera, Azadirachta indica, Benincasa hispida, Beta vulgaris, Caesalpinia bonducella, Citrullus colocynthis, Coccinia indica, Eucalyptus globules, Ficus bengalenesis, Gymnema sylvestre, Hibiscus rosasinesis, Ipomoea batatas, Jatropha curcus, Mangifera indica, Momordica charantia, Morus alba, Mucuna pruriens, Ocimum sanctum, Pterocarpus marsupium, Punica granatum, Syzigium cumini, Tinospora cordifolia, Trigonella foenum graecum. A wide array of plant derived active principles representing numerous chemical compounds has demonstrated activity consistent with their possible use in the treatment of diabetes.